Journal: Journal of Nanobiotechnology
Article Title: Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1
doi: 10.1186/s12951-020-00733-z
Figure Lengend Snippet: Characterization of TSC-derived exosomes. a The ultrastructure of exosomes was analyzed by transmission electron microscopy. b Western blot analysis showed the expression of protein markers of exosomes, such as CD63, CD9 and TSG101. c Representative DLS number distribution measurement of the isolated exosome population demonstrated an average diameter of 101 nm
Article Snippet: Then, the membranes were incubated with primary rabbit anti-mouse antibodies against CD9 (Cat: ab92726), CD63 (Cat: ab213090), TSG101 (Cat: ab125011), cleaved-caspase3 (Cat: ab2302), Bcl-2 (Cat: ab32124), Zeb1 (Cat: ab81972), p65 (Cat: ab16502) and GAPDH (Cat: ab181602) (Abcam; USA) at a dilution of 1:1000.
Techniques: Derivative Assay, Transmission Assay, Electron Microscopy, Western Blot, Expressing, Isolation